Application Note - Flow Scintillation Analyzer Interfaced with the HPLC and Nuclear Magnetic Resonance Spectrometer
نویسندگان
چکیده
Flow scintillation analysis is commonly used to quantify the radioisotope label on organic compounds such as biochemicals, drugs, and metabolites separated from complex mixtures by High Performance Liquid Chromatography (HPLC). The subsequent task of determining the molecular structure of the separated substances can be formidable. Traditional methods of structure determination involve collecting the HPLC separated fractions that correspond to activity peaks measured by the flow scintillation analyzer. The collected fractions are then isolated, further purified, and then submitted to spectroscopic methods of analysis such as mass spectrometry (MS) and Nuclear Magnetic Resonance (NMR) spectroscopy. Both MS and NMR methods provide complementary information that can be used to derive a molecular structure. Mass spectrometry can provide the molecular weight, molecular formula, and structure from ion fragmentation patterns, while NMR can provide additional important structural information including the spatial orientation of atoms in the molecular structure. A good example of this can be taken from early work of the author concerning the mass and NMR spectra of the inositol diastereomers, all of which produce virtually identical electron-impact mass spectra, but different NMR spectra (L’Annunziata, 1970 and L’Annunziata and Fuller, 1971 and 1976). A new and increasing popular approach to drug metabolism and natural product studies involves the direct measurement of the NMR spectra of compounds directly off the HPLC column obviating the need for compound isolation. This application note will describe new developments in linking the Packard Radiomatic flow scintillation analyzer from the HPLC to the NMR spectrometer to provide on-line (in-situ) molecular structure analysis of radioisotope-labeled compounds. Principle of NMR Spectroscopy
منابع مشابه
Flow Scintillation Analysis Application Note - Flow Scintillation Analyzer (FSA) Interfaced with the HPLC and Nuclear Magnetic Resonance (NMR) Spectrometer “A State-of-the-Art Application of the RadiomaticTM FSA”
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